human dermal fibroblast cell culture hdfa  (ATCC)

Journal: Biochemical and biophysical research communications

Article Title: A novel XBP1 variant is highly enriched in cancer tissues and is specifically required for cancer cell survival

doi: 10.1016/j.bbrc.2021.05.038

Figure Lengend Snippet: Xv1 is essential for survival of cancer cells (A-F) Caspase 3/7 activation in BT474 (A), HeLa (B), MCF10A (D), and human primary dermal fibroblast (HDFa) (E) cells transfected with siRNAs as indicated. Caspase 3/7 activity was monitored with fluorescence generated from cleavage of a caspase 3/7 substrate in an IncuCyte Live Cell Analysis System. The relative fluorescence unit (RFU) was calculated by normalizing the fluorescence intensity to cell confluence. n=12 (BT474 and MCF10A), n=20 (HeLa), n=6 (HDFa) independent fields per condition. Knockdown efficiencies were detected by RT-PCR (C and F). Note that Xv1 was not detected in MCF10A and HDFa cells due to their lacking Xv1 expression (see Fig. 2E). NCsi, negative control siRNA. (G) Caspase 3 and PARP-1 cleavage in Xv1 knockdown cells. Asterisks indicate the cleaved forms of caspase 3 and PARP-1. (H, I) Viability of BT474 (H) and HeLa (I) cells transfected with different siRNAs. Live cells were counted after trypan blue staining. n=3 repeats. (J) Transient expression of Xv1 rescues Xv1 knockdown-induced cell death. BT474 cells were first transfected with vector or siRNAs-resistant Xv1 cDNA and then received a second transfection with siRNAs as indicated. Caspase 3/7 activities were monitored as aforementioned. n=12 independent fields per condition.

Article Snippet: Cell culture Primary dermal fibroblast (HDFa) was purchased from ATCC (PCS-201-012).

Techniques: Activation Assay, Transfection, Activity Assay, Fluorescence, Generated, Cell Analysis, Knockdown, Reverse Transcription Polymerase Chain Reaction, Expressing, Negative Control, Staining, Plasmid Preparation

Journal: Journal of Materials Science. Materials in Medicine

Article Title: Tribological, biocompatibility, and antibiofilm properties of tungsten–germanium coating using magnetron sputtering

doi: 10.1007/s10856-020-06477-4

Figure Lengend Snippet: Immunofluorescence microscopy images of human fibroblast (HDFa) cell culture attachments on different surfaces after 24 h. a W–Ge coated borosilicate surface (×10 magnification), b W–Ge coated borosilicate surface (×20 magnification), c W–Ge coated borosilicate surface (×40 magnification), d cell culture plate bottom surface (×10 magnification), e cell culture plate bottom surface (×20 magnification), f cell culture plate bottom surface (×40 magnification), g uncoated borosilicate surface (×10 magnification), h uncoated borosilicate surface (×20 magnification), i uncoated borosilicate surface (×40 magnification)

Article Snippet: Human fibroblast (HDFa) cell culture (ATCC ® PCS201 012 TM ) was grown in T25 cell culture flask by using DMEM (high glucose 4.5 g/L, +glutamine, w/o pyruvate, Sigma ® , USA) included 10% FBS (Sigma ® , USA) and 1% Penicillin/streptomycin (10,000 IU/ml 10,000 μg/ml, Thermo Fisher Scientific ® , USA) at 37 °C, 5% CO 2 humidified incubator.

Techniques: Immunofluorescence, Microscopy, Cell Culture